北京快乐8玩法

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李国红课题组揭示RYBP-/YAF2-PRC1复合物和组蛋白H1介导染色质压缩协同促进H2AK119ub蔓延和继承的分子机制

发布时间: 2020年03月23日

  2020年3月23日,生物大分子国家重点实验室李国红课题组在Nature Cell Biology上发表了“RYBP/YAF2-PRC1 complexes and histone H1-dependent chromatin compaction mediate propagation of H2AK119ub1 during cell division”的文章。该研究系统阐述了组蛋白修饰H2AK119ub1在染色质上蔓延以及跨细胞周期继承的具体机制。

  zhenhexibaonei,ransezhishibiaoguanyichuanxinxidezhuyaodiaokongjiemian。zhuduobiaoguanyichuanyinzitongguodiaojieransezhijiegoudekaifangyuguanbi,kongzhilejiyindebiaodayuchenmo,congerweichilebutongpuxixibaoneijiyindechayibiaoda。qizhong,duoshuyizhifuhewu1(prc1)shiyileizhongyaodebiaoguanyichuandiaokongyinzi,tazhuyaotongguoyasuoransezhijiegouhecuihuaxingchengh2ak119ub1xiushizheiliangzhongtujingcujinlejianxingyiransezhidexingcheng,bingyiciweichileprc1bajiyindechenmozhuangtai[1]。h2ak119ub1zairansezhijubuwangwanglianmianbuduanxingchengfeichangkuanguangdeh2ak119ub1quyu,danshiqichanshengdejizhiyizhiweibeijiexi。

  liguohongketizujiehehexiaotipull-down、tiwaifansuhua、tetr-tetobaxianghechip-seqdengshiyanfaxianrybpheyaf2danbainengtongguoznfjiegouyuteyishibiebingjieheh2ak119ub1,jiecishirybp-prc1heyaf2-prc1fuhewuyuqicuihuachanwuzhijianxingchengsuoweide“mei-chanwuzhijiandezhengfankuijizhi”,congercujinleh2ak119ub1xiangzhoubiandemanyan。youqudeshi,tongguojiyuzudanbaixiushihednaseigaominweidianderansezhizhuangtaifenxi,zuozhefaxianh2ak119ub1zhuyaofenbuzaijiegoujinmideyiransezhiquyu,yincitichufeichangguiprc1jiedaodeh2ak119ub1manyanyekenengshoudaoransezhigaojijiegoudediaokong。zhiqian,liguohonghezhupingketizuhezuo,liyonglengdongdianjingjishujiexilezudanbaih1yasuo30nmransezhixianweidegaojingdujiegou,jieshiransezhixianweishiyisihexiaotiweijiegoudanyuandezuoshoushuangluoxuanjiegou,bingfaxianxiangjianhexiaotizhijiandexianghuzuoyongzaiweixisihexiaotineibuhesihexiaotizhijiandewendingxingzhongfahuilezhongyaozuoyong[2]。yinci,30nmransezhixianweikenengtongguohexiaoti-hexiaotipeidui(nucleosome-pairing)defangshicujinlefeichangguiprc1jiedaodeh2ak119ub1zairansezhixianweishangdemanyan。jiyugaijiashe,zuozhejinxinglejinyibudeshiyanbingfaxianh1yasuode30nmransezhixianweidequecujinleh2ak119ub1xianglinjinransezhiquyudemanyan。zaitiwaifansuhuashiyanzhong,zuozhetongguoyinruzudanbaixiangyingweizhidetubianti,zhengmingh1duih2ak119ub1manyandecujinzuoyongzhuyaotongguogaibianransezhigaojijiegoulaishixiande。bingqie,genju30nmransezhixianweidedianjingjiegou,zuozhetuicezheizhongcujinjizhikenengzunxunxiangjianhexiaoti-hexiaotidepeiduiyuanze[2]。

  zhongsuozhouzhi,dnafuzhiguochengzhongransezhihuijingliyici“pohuai-zhongjian”deguocheng。zaidnazilianshangxinhechengzudanbaidezhuangpeihuidaozhizudanbaixiushizhishaoxishiyibei[3, 4],ruguomeiyouyouxiaodezudanbaixiushihuifujizhi,xibaoshenfenhennandeyiweichi。neimeyishangjizhishifouyejiedaoleh2ak119ub1zaixibaozhouqidejichengni?liyongtetr-tetobaxiangxitong,zuozhefaxiandanchunbaxiangring1bzai8xtetofujinjinnengxingchenghenzhaideh2ak119ub1xiushi,dangliyongduoxihuansu(doxcycline,dox)xiquring1b-tetr-hashi,yiweixingchengdeh2ak119ub1xiushibingbunengzaizidaixibaoneidedaoyouxiaodeweichi。ranerguobiaodarybpcujinh2ak119ub1xiangliangcemanyanxingchengdayue10kbdequyushi,shiyanfaxianzhengfankuijizhinengjiedaoh2ak119ub1zai8xtetofujinweichizhishao6gexibaozhouqi。tongshi,zuozhefaxianh1jiedaoderansezhiyasuoduiyuh2ak119ub1deyichuanyezhiguanzhongyao。lingwaizaiquanjiyinzushang,zuozhefaxianrybptubianheh1c/h1d/h1esanqiaochudaozhideh2ak119ub1jiangdiyijijiyinbiaodabianhuayouhendachengdudezhongdie。bingqieliyongshenjingfenhuamoxing,zuozhefaxianrybptubianheh1c/h1d/h1esanqiaochujundaozhishenjingqiantixibaofenhuazhangai。yinci,shuomingrybp-/yaf2-prc1fuhewuhezudanbaih1tongguoxietongcujinxibaozhouqijianh2ak119ub1debosancanyulexibaofenhuaguocheng,tishigaibiaoguanyichuantonglujuyouzhongyaodeshengwuxuegongneng。

 

北京快乐8玩法tu1. feichangguiprc1heh1jiedaoxibaozhouqijianh2ak119ub1zaiyiransezhiquyudeyichuan

  gaigongzuodetongxunzuozheweishengwudafenziguojiazhongdianshiyanshideliguohongyanjiuyuan。liguohongketizudezhaojichenghewangminweibenwendegongtongdiyizuozhe,changluyuanheyujuanfuzelebenyanjiusuoshejideshengwuxinxifenxi。tongshigaiyanjiuhaidedaoshengwudafenziguojiazhongdianshiyanshizhubingyanjiuyuanjiqixueshengzhangtiantian、qinghuadaxueshenxiaohuajiaoshouhetianjinyikedaxuewuxudongjiaoshoudezhidaohebangzhu,bingdedaosuozhipupingtaiwangjifenghezhangmengmengyijiganranmianyipingtaiwangxiaoyandedalizhichi。

  wenzhanglianjie:

  cankaowenxian:

  【1】 gao, z., et al., pcgf homologs, cbx proteins, and rybp define functionally distinct prc1 family complexes. mol cell, 2012. 45(3): p. 344-56.

  【2】 song, f., et al., cryo-em study of the chromatin fiber reveals a double helix twisted by tetranucleosomal units. science, 2014. 344(6182): p. 376-80.

  【3】 jiang, d. and f. berger, dna replication-coupled histone modification maintains polycomb gene silencing in plants. science, 2017. 357(6356): p. 1146-1149.

  【4】 coleman, r.t. and g. struhl, causal role for inheritance of h3k27me3 in maintaining the off state of a drosophila hox gene. science, 2017. 356(6333).

 

(gonggao: liguohongyanjiuzu)

 

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